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vdac d73d12 antibody (Cell Signaling Technology Inc)

Name: vdac d73d12 antibody
Brand: Cell Signaling Technology Inc
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Cell Signaling Technology Inc vdac d73d12 antibody
$(a) Schematic illustration of NAD + biosynthetic pathways. In the de novo pathway the essential amino acid tryptophan (TRP) from the diet is utilized to produce NAD + via several reactions (dashed arrow). The salvage pathway recycles NAM, which is generated as a by-product of NAD + -dependent enzymes such as SIRT3; PA=Phthalic Acid. (b-h) BMMs were isolated from young female C57BL/6 mice and cultured with M-CSF and RANKL, in the presence or absence of FK866 or PA for 24 h. (b) NAD + levels in whole cells. (c) Representative images and quantification of TRAP-positive osteoclasts after 5 days. (d) NAD + levels in mitochondrial-enriched fractions. (e) SIRT3 activity. (f) Oxygen Consumption Rate. (g) ATP levels. (h) Apoptosis by caspase-3 activity. (i-j) Human BMMs were cultured with M-CSF and RANKL in the presence or absence of FK866, as above. (i) NAD + levels after 24 h and (j) representative images and quantification of TRAP-positive osteoclasts after 7 days. (k) mRNA levels of the indicated enzymes in BMMs cultured with M-CSF and RANKL the presence or absence of E 2 for the indicated timepoints. P values in grey indicate comparisons with time 0. P values in red indicate comparison of RANKL vs RANKL+E 2 within the same time point. (l) BMMs were isolated from young female C57BL/6 mice and cultured with M-CSF and RANKL the presence or absence of E 2 for 24 hours, representative Western blot images and quantification of the indicated proteins in mitochondrial enriched fractions; β-actin in cytosol and <t>VDAC</t> in mitochondria indicate efficacy of cellular compartment isolation. (m) mRNA levels in BMMs transduced with Nmnat1 <t>and</t> <t>Nmnat3</t> sh or control sh lentivirus particles. (n) NAD + levels after 24 h and (o) representative images and quantification of TRAP-positive osteoclasts after 5 days. Scale bar 500 µm. Line and error bars represent mean ± SD. P values using two-tailed Student’s t-test, 1-way ANOVA followed by Dunnett’s multiple comparisons test, or 2-way ANOVA followed by Šídák’s multiple comparisons test.$
Vdac D73d12 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vdac d73d12 antibody - by Bioz Stars, 2026-03

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1) Product Images from "Estrogens protect bone mass by inhibiting NAD + metabolism in osteoclasts"

Article Title: Estrogens protect bone mass by inhibiting NAD + metabolism in osteoclasts

Journal: bioRxiv

doi: 10.1101/2025.07.11.664289

$(a) Schematic illustration of NAD + biosynthetic pathways. In the de novo pathway the essential amino acid tryptophan (TRP) from the diet is utilized to produce NAD + via several reactions (dashed arrow). The salvage pathway recycles NAM, which is generated as a by-product of NAD + -dependent enzymes such as SIRT3; PA=Phthalic Acid. (b-h) BMMs were isolated from young female C57BL/6 mice and cultured with M-CSF and RANKL, in the presence or absence of FK866 or PA for 24 h. (b) NAD + levels in whole cells. (c) Representative images and quantification of TRAP-positive osteoclasts after 5 days. (d) NAD + levels in mitochondrial-enriched fractions. (e) SIRT3 activity. (f) Oxygen Consumption Rate. (g) ATP levels. (h) Apoptosis by caspase-3 activity. (i-j) Human BMMs were cultured with M-CSF and RANKL in the presence or absence of FK866, as above. (i) NAD + levels after 24 h and (j) representative images and quantification of TRAP-positive osteoclasts after 7 days. (k) mRNA levels of the indicated enzymes in BMMs cultured with M-CSF and RANKL the presence or absence of E 2 for the indicated timepoints. P values in grey indicate comparisons with time 0. P values in red indicate comparison of RANKL vs RANKL+E 2 within the same time point. (l) BMMs were isolated from young female C57BL/6 mice and cultured with M-CSF and RANKL the presence or absence of E 2 for 24 hours, representative Western blot images and quantification of the indicated proteins in mitochondrial enriched fractions; β-actin in cytosol and VDAC in mitochondria indicate efficacy of cellular compartment isolation. (m) mRNA levels in BMMs transduced with Nmnat1 and Nmnat3 sh or control sh lentivirus particles. (n) NAD + levels after 24 h and (o) representative images and quantification of TRAP-positive osteoclasts after 5 days. Scale bar 500 µm. Line and error bars represent mean ± SD. P values using two-tailed Student’s t-test, 1-way ANOVA followed by Dunnett’s multiple comparisons test, or 2-way ANOVA followed by Šídák’s multiple comparisons test.$
Figure Legend Snippet: (a) Schematic illustration of NAD + biosynthetic pathways. In the de novo pathway the essential amino acid tryptophan (TRP) from the diet is utilized to produce NAD + via several reactions (dashed arrow). The salvage pathway recycles NAM, which is generated as a by-product of NAD + -dependent enzymes such as SIRT3; PA=Phthalic Acid. (b-h) BMMs were isolated from young female C57BL/6 mice and cultured with M-CSF and RANKL, in the presence or absence of FK866 or PA for 24 h. (b) NAD + levels in whole cells. (c) Representative images and quantification of TRAP-positive osteoclasts after 5 days. (d) NAD + levels in mitochondrial-enriched fractions. (e) SIRT3 activity. (f) Oxygen Consumption Rate. (g) ATP levels. (h) Apoptosis by caspase-3 activity. (i-j) Human BMMs were cultured with M-CSF and RANKL in the presence or absence of FK866, as above. (i) NAD + levels after 24 h and (j) representative images and quantification of TRAP-positive osteoclasts after 7 days. (k) mRNA levels of the indicated enzymes in BMMs cultured with M-CSF and RANKL the presence or absence of E 2 for the indicated timepoints. P values in grey indicate comparisons with time 0. P values in red indicate comparison of RANKL vs RANKL+E 2 within the same time point. (l) BMMs were isolated from young female C57BL/6 mice and cultured with M-CSF and RANKL the presence or absence of E 2 for 24 hours, representative Western blot images and quantification of the indicated proteins in mitochondrial enriched fractions; β-actin in cytosol and VDAC in mitochondria indicate efficacy of cellular compartment isolation. (m) mRNA levels in BMMs transduced with Nmnat1 and Nmnat3 sh or control sh lentivirus particles. (n) NAD + levels after 24 h and (o) representative images and quantification of TRAP-positive osteoclasts after 5 days. Scale bar 500 µm. Line and error bars represent mean ± SD. P values using two-tailed Student’s t-test, 1-way ANOVA followed by Dunnett’s multiple comparisons test, or 2-way ANOVA followed by Šídák’s multiple comparisons test.

Techniques Used: Generated, Isolation, Cell Culture, Activity Assay, Comparison, Western Blot, Transduction, Control, Two Tailed Test

(a) Chemical reaction catalyzed by Lb NOX. (b) Western blot for FLAG, β-actin, and VDAC from BMMs cultured with M-CSF expressing the FLAG-tagged cytosolic and mitochondrial (mito) Lb NOX. β-actin in cytosol and VDAC in mitochondria indicate efficacy of cellular compartment isolation. (c) NAD + /NADH redox ratio and (d) ATP levels in BMMs expressing Lb NOX or mito Lb NOX. (e-g) BMMs expressing Lb NOX or mito Lb NOX cultured with M-CSF and RANKL in the presence or absence of E 2 (10 -8 M) for 24 hours. (e) NAD + /NADH redox ratio. (f) SIRT3 activity. (g) Representative images and quantification of TRAP-positive osteoclasts after 5 days. Scale bar 500 µm. Line and error bars represent mean ± SD. P values by 1-way ANOVA followed by Dunnett’s multiple comparisons test or 2-way ANOVA followed by Šídák’s multiple comparisons test.

Figure Legend Snippet: (a) Chemical reaction catalyzed by Lb NOX. (b) Western blot for FLAG, β-actin, and VDAC from BMMs cultured with M-CSF expressing the FLAG-tagged cytosolic and mitochondrial (mito) Lb NOX. β-actin in cytosol and VDAC in mitochondria indicate efficacy of cellular compartment isolation. (c) NAD + /NADH redox ratio and (d) ATP levels in BMMs expressing Lb NOX or mito Lb NOX. (e-g) BMMs expressing Lb NOX or mito Lb NOX cultured with M-CSF and RANKL in the presence or absence of E 2 (10 -8 M) for 24 hours. (e) NAD + /NADH redox ratio. (f) SIRT3 activity. (g) Representative images and quantification of TRAP-positive osteoclasts after 5 days. Scale bar 500 µm. Line and error bars represent mean ± SD. P values by 1-way ANOVA followed by Dunnett’s multiple comparisons test or 2-way ANOVA followed by Šídák’s multiple comparisons test.

Techniques Used: Western Blot, Cell Culture, Expressing, Isolation, Activity Assay

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Journal: bioRxiv

Article Title: Estrogens protect bone mass by inhibiting NAD + metabolism in osteoclasts

doi: 10.1101/2025.07.11.664289

Figure Lengend Snippet: (a) Schematic illustration of NAD + biosynthetic pathways. In the de novo pathway the essential amino acid tryptophan (TRP) from the diet is utilized to produce NAD + via several reactions (dashed arrow). The salvage pathway recycles NAM, which is generated as a by-product of NAD + -dependent enzymes such as SIRT3; PA=Phthalic Acid. (b-h) BMMs were isolated from young female C57BL/6 mice and cultured with M-CSF and RANKL, in the presence or absence of FK866 or PA for 24 h. (b) NAD + levels in whole cells. (c) Representative images and quantification of TRAP-positive osteoclasts after 5 days. (d) NAD + levels in mitochondrial-enriched fractions. (e) SIRT3 activity. (f) Oxygen Consumption Rate. (g) ATP levels. (h) Apoptosis by caspase-3 activity. (i-j) Human BMMs were cultured with M-CSF and RANKL in the presence or absence of FK866, as above. (i) NAD + levels after 24 h and (j) representative images and quantification of TRAP-positive osteoclasts after 7 days. (k) mRNA levels of the indicated enzymes in BMMs cultured with M-CSF and RANKL the presence or absence of E 2 for the indicated timepoints. P values in grey indicate comparisons with time 0. P values in red indicate comparison of RANKL vs RANKL+E 2 within the same time point. (l) BMMs were isolated from young female C57BL/6 mice and cultured with M-CSF and RANKL the presence or absence of E 2 for 24 hours, representative Western blot images and quantification of the indicated proteins in mitochondrial enriched fractions; β-actin in cytosol and VDAC in mitochondria indicate efficacy of cellular compartment isolation. (m) mRNA levels in BMMs transduced with Nmnat1 and Nmnat3 sh or control sh lentivirus particles. (n) NAD + levels after 24 h and (o) representative images and quantification of TRAP-positive osteoclasts after 5 days. Scale bar 500 µm. Line and error bars represent mean ± SD. P values using two-tailed Student’s t-test, 1-way ANOVA followed by Dunnett’s multiple comparisons test, or 2-way ANOVA followed by Šídák’s multiple comparisons test.

Article Snippet: The following primary monoclonal antibodies were used: NAMPT (Abcam, ab236874, 1:1000); NMNAT3 (Santa Cruz, sc-390433, 1:100); FLAG (Cell Signaling; 2368; 1:1000); VDAC (Cell Signaling; D73D12; 1:1000) and β-actin (Santa Cruz; sc-47778; 1:5000).

Techniques: Generated, Isolation, Cell Culture, Activity Assay, Comparison, Western Blot, Transduction, Control, Two Tailed Test

Journal: bioRxiv

Article Title: Estrogens protect bone mass by inhibiting NAD + metabolism in osteoclasts

doi: 10.1101/2025.07.11.664289

Figure Lengend Snippet: (a) Chemical reaction catalyzed by Lb NOX. (b) Western blot for FLAG, β-actin, and VDAC from BMMs cultured with M-CSF expressing the FLAG-tagged cytosolic and mitochondrial (mito) Lb NOX. β-actin in cytosol and VDAC in mitochondria indicate efficacy of cellular compartment isolation. (c) NAD + /NADH redox ratio and (d) ATP levels in BMMs expressing Lb NOX or mito Lb NOX. (e-g) BMMs expressing Lb NOX or mito Lb NOX cultured with M-CSF and RANKL in the presence or absence of E 2 (10 -8 M) for 24 hours. (e) NAD + /NADH redox ratio. (f) SIRT3 activity. (g) Representative images and quantification of TRAP-positive osteoclasts after 5 days. Scale bar 500 µm. Line and error bars represent mean ± SD. P values by 1-way ANOVA followed by Dunnett’s multiple comparisons test or 2-way ANOVA followed by Šídák’s multiple comparisons test.

Techniques: Western Blot, Cell Culture, Expressing, Isolation, Activity Assay

Journal: iScience

Article Title: Prenatal hormone stress triggers embryonic cardiac hypertrophy outcome by ubiquitin-dependent degradation of mitochondrial mitofusin 2

doi: 10.1016/j.isci.2023.108690

Figure Lengend Snippet:

Article Snippet: Rabbit monoclonal anti-VDAC (D73D12) , Cell Signaling Technology , Cat#: 4661 RRID: AB_10557420.

Techniques: Ubiquitin Proteomics, Recombinant, Protease Inhibitor, Lysis, Western Blot, ATP Assay, Isolation, Staining, Modification, Bicinchoninic Acid Protein Assay, Sequencing, Plasmid Preparation, Software